bca (Thermo Fisher)
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 
99
Structured Review
Thermo Fisher
bca
Bca, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bca+protein+assay+kit/BCA+Protein+Assay+Kit/pmc13316761-65-7-13
Average 99 stars, based on 1 article reviews
Bca, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bca+protein+assay+kit/BCA+Protein+Assay+Kit/pmc13316761-65-7-13
Average 99 stars, based on 1 article reviews
bca - by Bioz Stars,
2026-10
99/100 stars
Images
Related Articles
Protein Concentration:Article Title: Title Pending 17760 Article Snippet: This study investigated the effect of freezing and subsequent aging on beef quality, particularly focusing on the extent of postmortem proteolysis and tenderization.. The longissimus lumborum muscle was collected from 8 steers 24 h postmortem, sliced into 8 2.5-cm-thick steaks, and randomly allocated into 4 groups.. Treatment groups consisted of 1) aging at 4°C for 24 h; 2) aging for 168 h; 3) freezing at −20°C for 24 h followed by thawing/aging for 24 h; and 4) freezing for 24 h followed by thawing/aging for 168 h. In general, freezing decreased the color intensity of the steaks, whereas aging increased it (P< 0.05). Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Bicinchoninic Acid Protein Assay:Article Title: Title Pending 17760 Article Snippet: This study investigated the effect of freezing and subsequent aging on beef quality, particularly focusing on the extent of postmortem proteolysis and tenderization.. The longissimus lumborum muscle was collected from 8 steers 24 h postmortem, sliced into 8 2.5-cm-thick steaks, and randomly allocated into 4 groups.. Treatment groups consisted of 1) aging at 4°C for 24 h; 2) aging for 168 h; 3) freezing at −20°C for 24 h followed by thawing/aging for 24 h; and 4) freezing for 24 h followed by thawing/aging for 168 h. In general, freezing decreased the color intensity of the steaks, whereas aging increased it (P< 0.05). Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle Article Snippet: .. Protein content of the supernatants was determined by Co-Immunoprecipitation Assay:Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Lysis:Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease. Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by Incubation:Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce BIA-KA:Article Title: Polymeric alpha-1 antitrypsin perturbs Paneth cell proteostasis and exacerbates intestinal inflammation. Article Snippet: .. Coimmunoprecipitation assay Intestinal crypt lysates were prepared in cold RIPA lysis buffer (50 mM Tris-HCl, 150mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.1% SDS, pH 7.4) in the presence of protease and phosphatase inhibitors, incubated for 30 min on ice, and centrifuged at 16,000 rcf for 15 min. Supernatants were collected, and the protein concentration was determined with a Pierce Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease. Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events. Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce Article Title: Bioinspired nanoformulation for BRD4 protein degradation in aggressive HER2+ breast cancer Article Snippet: reagent), Trizma base solution, sodium acetate (anhydrous), acetic acid (glacial, ≥99%), phosphate buffered saline (PBS, 0.01 M), potassium bromide (FT-IR grade), Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS, USA origin), methyl thiazolyltetrazolium (MTT reagent), and rhodamine B (RhB) were obtained from Sigma Aldrich (St. Louis, MO, USA). .. Pierce Article Title: A fraction of diet-derived microRNAs present in plant extracellular vesicles remain detectable after gastrointestinal digestion and shows epithelial uptake in a CaCo-2 cell model. Article Snippet: Chemicals were purchased from Sigma Aldrich (St. Louis, MO, US), including KCl, KH2PO4, NaHCO3, NaCl, MgCl2(H2O)6, (NH4)2CO3, CaCl2(H2O)2, porcine pepsin from gastric mucosa (powder, 250 U/mg, Cat. P7000), porcine pancreatin (activity equivalent to 4× USP specifications, Cat B1750), bovine bile (B3883), as well as chloroform, isopropanol 99.5%, and ethanol 100%. .. mirVana mimics (cel-miR39-3p and bol-miR172a), Lipofectamine 3000, Qubit RNA High Sensitivity Assay kit, Qubit microRNA Assay kit, Pierce Cell-Signaling:Article Title: RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease. Article Snippet: .. Cell lysis buffer contained 30 mM HEPES, pH 7.4, 100 mM NaCl, 1% Triton X-100, 20 mM NaF, 1 mM EGTA, 1 mM Na3V_O4 (Cell Signaling), 1 mM PMSF (Bioshop), protease inhibitors (Roche), phosphatase inhibitors (PhosSTOP, Roche); and protein content was measured by Sonication:Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events. Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce Centrifugation:Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events. Article Snippet: .. The resulting pellet was lysed in IPH buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 5 mM EDTA; 0.5% NP-40; 10% glycerol; with protease and phosphatase inhibitors) and sonicated with the Qsonica Q800R2 system (Qsonica) at 70% amplitude 30sec on 30sec off setting for 20 min. Lysates were clarified by centrifugation at maximum speed for 10 min at 4 ◦C and protein concentrations determined using the Pierce Qubit:Article Title: A fraction of diet-derived microRNAs present in plant extracellular vesicles remain detectable after gastrointestinal digestion and shows epithelial uptake in a CaCo-2 cell model. Article Snippet: Chemicals were purchased from Sigma Aldrich (St. Louis, MO, US), including KCl, KH2PO4, NaHCO3, NaCl, MgCl2(H2O)6, (NH4)2CO3, CaCl2(H2O)2, porcine pepsin from gastric mucosa (powder, 250 U/mg, Cat. P7000), porcine pancreatin (activity equivalent to 4× USP specifications, Cat B1750), bovine bile (B3883), as well as chloroform, isopropanol 99.5%, and ethanol 100%. .. mirVana mimics (cel-miR39-3p and bol-miR172a), Lipofectamine 3000, Qubit RNA High Sensitivity Assay kit, Qubit microRNA Assay kit, Pierce Sensitivity:Article Title: A fraction of diet-derived microRNAs present in plant extracellular vesicles remain detectable after gastrointestinal digestion and shows epithelial uptake in a CaCo-2 cell model. Article Snippet: Chemicals were purchased from Sigma Aldrich (St. Louis, MO, US), including KCl, KH2PO4, NaHCO3, NaCl, MgCl2(H2O)6, (NH4)2CO3, CaCl2(H2O)2, porcine pepsin from gastric mucosa (powder, 250 U/mg, Cat. P7000), porcine pancreatin (activity equivalent to 4× USP specifications, Cat B1750), bovine bile (B3883), as well as chloroform, isopropanol 99.5%, and ethanol 100%. .. mirVana mimics (cel-miR39-3p and bol-miR172a), Lipofectamine 3000, Qubit RNA High Sensitivity Assay kit, Qubit microRNA Assay kit, Pierce Modification:Article Title: A fraction of diet-derived microRNAs present in plant extracellular vesicles remain detectable after gastrointestinal digestion and shows epithelial uptake in a CaCo-2 cell model. Article Snippet: Chemicals were purchased from Sigma Aldrich (St. Louis, MO, US), including KCl, KH2PO4, NaHCO3, NaCl, MgCl2(H2O)6, (NH4)2CO3, CaCl2(H2O)2, porcine pepsin from gastric mucosa (powder, 250 U/mg, Cat. P7000), porcine pancreatin (activity equivalent to 4× USP specifications, Cat B1750), bovine bile (B3883), as well as chloroform, isopropanol 99.5%, and ethanol 100%. .. mirVana mimics (cel-miR39-3p and bol-miR172a), Lipofectamine 3000, Qubit RNA High Sensitivity Assay kit, Qubit microRNA Assay kit, Pierce Sterility:Article Title: A fraction of diet-derived microRNAs present in plant extracellular vesicles remain detectable after gastrointestinal digestion and shows epithelial uptake in a CaCo-2 cell model. Article Snippet: Chemicals were purchased from Sigma Aldrich (St. Louis, MO, US), including KCl, KH2PO4, NaHCO3, NaCl, MgCl2(H2O)6, (NH4)2CO3, CaCl2(H2O)2, porcine pepsin from gastric mucosa (powder, 250 U/mg, Cat. P7000), porcine pancreatin (activity equivalent to 4× USP specifications, Cat B1750), bovine bile (B3883), as well as chloroform, isopropanol 99.5%, and ethanol 100%. .. mirVana mimics (cel-miR39-3p and bol-miR172a), Lipofectamine 3000, Qubit RNA High Sensitivity Assay kit, Qubit microRNA Assay kit, Pierce Positron Emission Tomography:Article Title: A fraction of diet-derived microRNAs present in plant extracellular vesicles remain detectable after gastrointestinal digestion and shows epithelial uptake in a CaCo-2 cell model. Article Snippet: Chemicals were purchased from Sigma Aldrich (St. Louis, MO, US), including KCl, KH2PO4, NaHCO3, NaCl, MgCl2(H2O)6, (NH4)2CO3, CaCl2(H2O)2, porcine pepsin from gastric mucosa (powder, 250 U/mg, Cat. P7000), porcine pancreatin (activity equivalent to 4× USP specifications, Cat B1750), bovine bile (B3883), as well as chloroform, isopropanol 99.5%, and ethanol 100%. .. mirVana mimics (cel-miR39-3p and bol-miR172a), Lipofectamine 3000, Qubit RNA High Sensitivity Assay kit, Qubit microRNA Assay kit, Pierce Membrane:Article Title: A fraction of diet-derived microRNAs present in plant extracellular vesicles remain detectable after gastrointestinal digestion and shows epithelial uptake in a CaCo-2 cell model. Article Snippet: Chemicals were purchased from Sigma Aldrich (St. Louis, MO, US), including KCl, KH2PO4, NaHCO3, NaCl, MgCl2(H2O)6, (NH4)2CO3, CaCl2(H2O)2, porcine pepsin from gastric mucosa (powder, 250 U/mg, Cat. P7000), porcine pancreatin (activity equivalent to 4× USP specifications, Cat B1750), bovine bile (B3883), as well as chloroform, isopropanol 99.5%, and ethanol 100%. .. mirVana mimics (cel-miR39-3p and bol-miR172a), Lipofectamine 3000, Qubit RNA High Sensitivity Assay kit, Qubit microRNA Assay kit, Pierce |